In vitro Cell Migration, Invasion, and Adhesion Assays: From Cell Imaging to Data Analysis
Identifieur interne : 000B62 ( Main/Exploration ); précédent : 000B61; suivant : 000B63In vitro Cell Migration, Invasion, and Adhesion Assays: From Cell Imaging to Data Analysis
Auteurs : Jordi Pijuan [Espagne] ; Carla Barcel [Espagne] ; David F. Moreno [Espagne] ; Oscar Maiques [Espagne] ; Pol Sis [Espagne] ; Rosa M. Marti [Espagne] ; Anna Macià [Espagne] ; Anaïs Panosa [Espagne]Source :
- Frontiers in Cell and Developmental Biology [ 2296-634X ] ; 2019.
Abstract
Cell migration is a key procedure involved in many biological processes including embryological development, tissue formation, immune defense or inflammation, and cancer progression. How physical, chemical, and molecular aspects can affect cell motility is a challenge to understand migratory cells behavior.
Url:
DOI: 10.3389/fcell.2019.00107
PubMed: 31259172
PubMed Central: 6587234
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000341
- to stream Pmc, to step Curation: 000341
- to stream Pmc, to step Checkpoint: 000A85
- to stream Ncbi, to step Merge: 000742
- to stream Ncbi, to step Curation: 000742
- to stream Ncbi, to step Checkpoint: 000742
- to stream Main, to step Merge: 000B62
- to stream Main, to step Curation: 000B62
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en"><italic>In vitro</italic> Cell Migration, Invasion, and Adhesion Assays: From Cell Imaging to Data Analysis</title><author><name sortKey="Pijuan, Jordi" sort="Pijuan, Jordi" uniqKey="Pijuan J" first="Jordi" last="Pijuan">Jordi Pijuan</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Flow Cytometry and Confocal Microscopy Unit, IRBLleida, University of Lleida</institution>,<addr-line>Lleida</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Barcel, Carla" sort="Barcel, Carla" uniqKey="Barcel C" first="Carla" last="Barcel">Carla Barcel</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>IRBLleida, University of Lleida</institution>,<addr-line>Lleida</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Moreno, David F" sort="Moreno, David F" uniqKey="Moreno D" first="David F." last="Moreno">David F. Moreno</name><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Molecular Biology Institute of Barcelona, CSIC</institution>,<addr-line>Barcelona</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Maiques, Oscar" sort="Maiques, Oscar" uniqKey="Maiques O" first="Oscar" last="Maiques">Oscar Maiques</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>IRBLleida, University of Lleida</institution>,<addr-line>Lleida</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Sis, Pol" sort="Sis, Pol" uniqKey="Sis P" first="Pol" last="Sis">Pol Sis</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>IRBLleida, University of Lleida</institution>,<addr-line>Lleida</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Marti, Rosa M" sort="Marti, Rosa M" uniqKey="Marti R" first="Rosa M." last="Marti">Rosa M. Marti</name><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Department of Dermatology, Hospital Universitari Arnau de Vilanova, University of Lleida, IRBLleida</institution>,<addr-line>Lleida</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Center of Biomedical Research on Cancer (CIBERONC), Instituto de Salud Carlos III</institution>,<addr-line>Madrid</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Macia, Anna" sort="Macia, Anna" uniqKey="Macia A" first="Anna" last="Macià">Anna Macià</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>IRBLleida, University of Lleida</institution>,<addr-line>Lleida</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Panosa, Anais" sort="Panosa, Anais" uniqKey="Panosa A" first="Anaïs" last="Panosa">Anaïs Panosa</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Flow Cytometry and Confocal Microscopy Unit, IRBLleida, University of Lleida</institution>,<addr-line>Lleida</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31259172</idno><idno type="pmc">6587234</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587234</idno><idno type="RBID">PMC:6587234</idno><idno type="doi">10.3389/fcell.2019.00107</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000341</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000341</idno><idno type="wicri:Area/Pmc/Curation">000341</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000341</idno><idno type="wicri:Area/Pmc/Checkpoint">000A85</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000A85</idno><idno type="wicri:Area/Ncbi/Merge">000742</idno><idno type="wicri:Area/Ncbi/Curation">000742</idno><idno type="wicri:Area/Ncbi/Checkpoint">000742</idno><idno type="wicri:Area/Main/Merge">000B62</idno><idno type="wicri:Area/Main/Curation">000B62</idno><idno type="wicri:Area/Main/Exploration">000B62</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>In vitro</italic> Cell Migration, Invasion, and Adhesion Assays: From Cell Imaging to Data Analysis</title><author><name sortKey="Pijuan, Jordi" sort="Pijuan, Jordi" uniqKey="Pijuan J" first="Jordi" last="Pijuan">Jordi Pijuan</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Flow Cytometry and Confocal Microscopy Unit, IRBLleida, University of Lleida</institution>,<addr-line>Lleida</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Barcel, Carla" sort="Barcel, Carla" uniqKey="Barcel C" first="Carla" last="Barcel">Carla Barcel</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>IRBLleida, University of Lleida</institution>,<addr-line>Lleida</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Moreno, David F" sort="Moreno, David F" uniqKey="Moreno D" first="David F." last="Moreno">David F. Moreno</name><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>Molecular Biology Institute of Barcelona, CSIC</institution>,<addr-line>Barcelona</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Maiques, Oscar" sort="Maiques, Oscar" uniqKey="Maiques O" first="Oscar" last="Maiques">Oscar Maiques</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>IRBLleida, University of Lleida</institution>,<addr-line>Lleida</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Sis, Pol" sort="Sis, Pol" uniqKey="Sis P" first="Pol" last="Sis">Pol Sis</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>IRBLleida, University of Lleida</institution>,<addr-line>Lleida</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Marti, Rosa M" sort="Marti, Rosa M" uniqKey="Marti R" first="Rosa M." last="Marti">Rosa M. Marti</name><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>Department of Dermatology, Hospital Universitari Arnau de Vilanova, University of Lleida, IRBLleida</institution>,<addr-line>Lleida</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Center of Biomedical Research on Cancer (CIBERONC), Instituto de Salud Carlos III</institution>,<addr-line>Madrid</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Macia, Anna" sort="Macia, Anna" uniqKey="Macia A" first="Anna" last="Macià">Anna Macià</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>IRBLleida, University of Lleida</institution>,<addr-line>Lleida</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Panosa, Anais" sort="Panosa, Anais" uniqKey="Panosa A" first="Anaïs" last="Panosa">Anaïs Panosa</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>Flow Cytometry and Confocal Microscopy Unit, IRBLleida, University of Lleida</institution>,<addr-line>Lleida</addr-line>,<country>Spain</country></nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Frontiers in Cell and Developmental Biology</title><idno type="eISSN">2296-634X</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Cell migration is a key procedure involved in many biological processes including embryological development, tissue formation, immune defense or inflammation, and cancer progression. How physical, chemical, and molecular aspects can affect cell motility is a challenge to understand migratory cells behavior. <italic>In vitro</italic> assays are excellent approaches to extrapolate to <italic>in vivo</italic> situations and study live cells behavior. Here we present four <italic>in vitro</italic> protocols that describe step-by-step cell migration, invasion and adhesion strategies and their corresponding image data quantification. These current protocols are based on <italic>two-dimensional</italic> wound healing assays (comparing traditional pipette tip-scratch assay vs. culture insert assay), 2D individual cell-tracking experiments by live cell imaging and <italic>three-dimensional</italic> spreading and transwell assays. All together, they cover different phenotypes and hallmarks of cell motility and adhesion, providing orthogonal information that can be used either individually or collectively in many different experimental setups. These optimized protocols will facilitate physiological and cellular characterization of these processes, which may be used for fast screening of specific therapeutic cancer drugs for migratory function, novel strategies in cancer diagnosis, and for assaying new molecules involved in adhesion and invasion metastatic properties of cancer cells.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Aman, A" uniqKey="Aman A">A. Aman</name></author><author><name sortKey="Piotrowski, T" uniqKey="Piotrowski T">T. Piotrowski</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Anderson, R L" uniqKey="Anderson R">R. L. Anderson</name></author><author><name sortKey="Balasas, T" uniqKey="Balasas T">T. Balasas</name></author><author><name sortKey="Callaghan, J" uniqKey="Callaghan J">J. Callaghan</name></author><author><name sortKey="Coombes, R C" uniqKey="Coombes R">R. C. Coombes</name></author><author><name sortKey="Evans, J" uniqKey="Evans J">J. Evans</name></author><author><name sortKey="Hall, J A" uniqKey="Hall J">J. A. Hall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Boya, P" uniqKey="Boya P">P. Boya</name></author><author><name sortKey="Gonzalez Polo, R A" uniqKey="Gonzalez Polo R">R.-A. Gonzalez-Polo</name></author><author><name sortKey="Casares, N" uniqKey="Casares N">N. Casares</name></author><author><name sortKey="Perfettini, J L" uniqKey="Perfettini J">J.-L. Perfettini</name></author><author><name sortKey="Dessen, P" uniqKey="Dessen P">P. Dessen</name></author><author><name sortKey="Larochette, N" uniqKey="Larochette N">N. Larochette</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Charras, G" uniqKey="Charras G">G. Charras</name></author><author><name sortKey="Sahai, E" uniqKey="Sahai E">E. Sahai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Pascalis, C" uniqKey="De Pascalis C">C. De Pascalis</name></author><author><name sortKey="Etienne Manneville, S" uniqKey="Etienne Manneville S">S. Etienne-Manneville</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Farina, K L" uniqKey="Farina K">K. L. Farina</name></author><author><name sortKey="Wyckoff, J B" uniqKey="Wyckoff J">J. B. Wyckoff</name></author><author><name sortKey="Rivera, J" uniqKey="Rivera J">J. Rivera</name></author><author><name sortKey="Lee, H" uniqKey="Lee H">H. Lee</name></author><author><name sortKey="Segall, J E" uniqKey="Segall J">J. E. Segall</name></author><author><name sortKey="Condeelis, J S" uniqKey="Condeelis J">J. S. Condeelis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Friedl, P" uniqKey="Friedl P">P. Friedl</name></author><author><name sortKey="Alexander, S" uniqKey="Alexander S">S. Alexander</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Friedl, P" uniqKey="Friedl P">P. Friedl</name></author><author><name sortKey="Weigelin, B" uniqKey="Weigelin B">B. Weigelin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Friedl, P" uniqKey="Friedl P">P. Friedl</name></author><author><name sortKey="Wolf, K" uniqKey="Wolf K">K. Wolf</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Icha, J" uniqKey="Icha J">J. Icha</name></author><author><name sortKey="Weber, M" uniqKey="Weber M">M. Weber</name></author><author><name sortKey="Waters, J C" uniqKey="Waters J">J. C. Waters</name></author><author><name sortKey="Norden, C" uniqKey="Norden C">C. Norden</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kramer, N" uniqKey="Kramer N">N. Kramer</name></author><author><name sortKey="Walzl, A" uniqKey="Walzl A">A. Walzl</name></author><author><name sortKey="Unger, C" uniqKey="Unger C">C. Unger</name></author><author><name sortKey="Rosner, M" uniqKey="Rosner M">M. Rosner</name></author><author><name sortKey="Krupitza, G" uniqKey="Krupitza G">G. Krupitza</name></author><author><name sortKey="Hengstschl Ger, M" uniqKey="Hengstschl Ger M">M. Hengstschläger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Macia, A" uniqKey="Macia A">A. Macià</name></author><author><name sortKey="Herreros, J" uniqKey="Herreros J">J. Herreros</name></author><author><name sortKey="Marti, R M" uniqKey="Marti R">R. M. Martí</name></author><author><name sortKey="Canti, C" uniqKey="Canti C">C. Cantí</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maiques, O" uniqKey="Maiques O">O. Maiques</name></author><author><name sortKey="Barcel, C" uniqKey="Barcel C">C. Barceló</name></author><author><name sortKey="Panosa, A" uniqKey="Panosa A">A. Panosa</name></author><author><name sortKey="Pijuan, J" uniqKey="Pijuan J">J. Pijuan</name></author><author><name sortKey="Orgaz, J L" uniqKey="Orgaz J">J. L. Orgaz</name></author><author><name sortKey="Rodriguez Hernandez, I" uniqKey="Rodriguez Hernandez I">I. Rodriguez-Hernandez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mayor, R" uniqKey="Mayor R">R. Mayor</name></author><author><name sortKey="Etienne Manneville, S" uniqKey="Etienne Manneville S">S. Etienne-Manneville</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ridley, A J" uniqKey="Ridley A">A. J. Ridley</name></author><author><name sortKey="Schwartz, M A" uniqKey="Schwartz M">M. A. Schwartz</name></author><author><name sortKey="Burridge, K" uniqKey="Burridge K">K. Burridge</name></author><author><name sortKey="Firtel, R A" uniqKey="Firtel R">R. A. Firtel</name></author><author><name sortKey="Ginsberg, M H" uniqKey="Ginsberg M">M. H. Ginsberg</name></author><author><name sortKey="Borisy, G" uniqKey="Borisy G">G. Borisy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="R Rth, P" uniqKey="R Rth P">P. Rørth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Salvatierra, E" uniqKey="Salvatierra E">E. Salvatierra</name></author><author><name sortKey="Alvarez, M J" uniqKey="Alvarez M">M. J. Alvarez</name></author><author><name sortKey="Leishman, C C" uniqKey="Leishman C">C. C. Leishman</name></author><author><name sortKey="Rivas Baquero, E" uniqKey="Rivas Baquero E">E. Rivas Baquero</name></author><author><name sortKey="Lutzky, V P" uniqKey="Lutzky V">V. P. Lutzky</name></author><author><name sortKey="Chuluyan, H E" uniqKey="Chuluyan H">H. E. Chuluyan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stuelten, C H" uniqKey="Stuelten C">C. H. Stuelten</name></author><author><name sortKey="Parent, C A" uniqKey="Parent C">C. A. Parent</name></author><author><name sortKey="Montell, D J" uniqKey="Montell D">D. J. Montell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Te Boekhorst, V" uniqKey="Te Boekhorst V">V. te Boekhorst</name></author><author><name sortKey="Preziosi, L" uniqKey="Preziosi L">L. Preziosi</name></author><author><name sortKey="Friedl, P" uniqKey="Friedl P">P. Friedl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Trepat, X" uniqKey="Trepat X">X. Trepat</name></author><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z. Chen</name></author><author><name sortKey="Jacobson, K" uniqKey="Jacobson K">K. Jacobson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Helvert, S" uniqKey="Van Helvert S">S. van Helvert</name></author><author><name sortKey="Storm, C" uniqKey="Storm C">C. Storm</name></author><author><name sortKey="Friedl, P" uniqKey="Friedl P">P. Friedl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, X" uniqKey="Wang X">X. Wang</name></author><author><name sortKey="Lao, Y" uniqKey="Lao Y">Y. Lao</name></author><author><name sortKey="Xu, N" uniqKey="Xu N">N. Xu</name></author><author><name sortKey="Xi, Z" uniqKey="Xi Z">Z. Xi</name></author><author><name sortKey="Wu, M" uniqKey="Wu M">M. Wu</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, L" uniqKey="Xu L">L. Xu</name></author><author><name sortKey="Gordon, R" uniqKey="Gordon R">R. Gordon</name></author><author><name sortKey="Farmer, R" uniqKey="Farmer R">R. Farmer</name></author><author><name sortKey="Pattanayak, A" uniqKey="Pattanayak A">A. Pattanayak</name></author><author><name sortKey="Binkowski, A" uniqKey="Binkowski A">A. Binkowski</name></author><author><name sortKey="Huang, X" uniqKey="Huang X">X. Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, Y" uniqKey="Zhu Y">Y. Zhu</name></author><author><name sortKey="Ye, T" uniqKey="Ye T">T. Ye</name></author><author><name sortKey="Yu, X" uniqKey="Yu X">X. Yu</name></author><author><name sortKey="Lei, Q" uniqKey="Lei Q">Q. Lei</name></author><author><name sortKey="Yang, F" uniqKey="Yang F">F. Yang</name></author><author><name sortKey="Xia, Y" uniqKey="Xia Y">Y. Xia</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Espagne</li></country></list><tree><country name="Espagne"><noRegion><name sortKey="Pijuan, Jordi" sort="Pijuan, Jordi" uniqKey="Pijuan J" first="Jordi" last="Pijuan">Jordi Pijuan</name></noRegion><name sortKey="Barcel, Carla" sort="Barcel, Carla" uniqKey="Barcel C" first="Carla" last="Barcel">Carla Barcel</name><name sortKey="Macia, Anna" sort="Macia, Anna" uniqKey="Macia A" first="Anna" last="Macià">Anna Macià</name><name sortKey="Maiques, Oscar" sort="Maiques, Oscar" uniqKey="Maiques O" first="Oscar" last="Maiques">Oscar Maiques</name><name sortKey="Marti, Rosa M" sort="Marti, Rosa M" uniqKey="Marti R" first="Rosa M." last="Marti">Rosa M. Marti</name><name sortKey="Marti, Rosa M" sort="Marti, Rosa M" uniqKey="Marti R" first="Rosa M." last="Marti">Rosa M. Marti</name><name sortKey="Moreno, David F" sort="Moreno, David F" uniqKey="Moreno D" first="David F." last="Moreno">David F. Moreno</name><name sortKey="Panosa, Anais" sort="Panosa, Anais" uniqKey="Panosa A" first="Anaïs" last="Panosa">Anaïs Panosa</name><name sortKey="Sis, Pol" sort="Sis, Pol" uniqKey="Sis P" first="Pol" last="Sis">Pol Sis</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B62 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000B62 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:6587234
|texte= In vitro Cell Migration, Invasion, and Adhesion Assays: From Cell Imaging to Data Analysis
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31259172" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ChloroquineV1
| This area was generated with Dilib version V0.6.33. |  |